ALPL ELISA Kit

Catalog No. ABIN5520576

Human ALPL ELISA Kit Colorimetric assay for quantification of Human ALPL and has been mentioned in 1 publication.

Quick Overview for ALPL ELISA Kit (ABIN5520576)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.125 ng/mL - 200 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details ALPL ELISA Kit

Minimum Detection Limit: 3.125 ng/mL

Purpose: For quantitative detection of BALP in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of BALP. No significant cross-reactivity or interference between BALP and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between BALP and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 1.875 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for ALPL ELISA Kit (ABIN5520576)

Popko, Karpiński, Chojnowska, Maresz, Milewski, Badmaev, Schurgers: "Decreased Levels of Circulating Carboxylated Osteocalcin in Children with Low Energy Fractures: A Pilot Study." in: Nutrients, Vol. 10, Issue 6, (2018) (PubMed).

Target Details for ALPL

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: BALP

Background: BALP(Bone Alkaline Phosphatase)/ALPL/BSAP/HOPS/AP-TNAP/TNSALP